CD74 antibody (FITC)

Catalog No. ABIN316495

The Mouse Monoclonal anti-CD74 antibody has been validated for FACS. It is suitable to detect CD74 in samples from Human.

Quick Overview for CD74 antibody (FITC) (ABIN316495)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: Bu45

Product Details anti-CD74 Antibody (FITC)

Specificity: This antibody recognises CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Bu45 antibody recognises 33, 35 and 41 kDa isoforms of CD74. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 Molecule, which is distal from the C-terminus. *Membrane permeabilisation may enhance CD74 staining on monocytes but is not required when staining lymphocytes.

Characteristics: Synonyms: p33, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DRantigens-associated invariant chain, Ia antigen-associated invariant chain

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: B lymphoblastoid cell line HFB1.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/5, 10l of the suggested working dilution to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide (NaN3), 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Background: The CD74 Molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes.Synonyms: DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, p33

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints